The Queen Bee is one of the main heroes of the 1950 French film, "Johnny the Giant Killer"
Johnny and a troop of boy scouts are camping in a forest. One night Johnny reads a tentful of scouts a fairy tale book about the evil giant. They sneak out to find the giant’s castle. The giant captures them all except for Johnny, whom he shrinks to insect size. A bird carries Johnny from the castle to the forest, where he sees the friendly insects of Insect-ville, saves them from a lizard, is captured by spiders, and is rescued by bees. A bee majordomo shows him how bees live. The queen bee favors Johnny, which makes the drones jealous. After Johnny defeats the head drone in a sword fight, the drone sneaks out to help the evil wasps to capture the beehive. Johnny defeats them, and the grateful queen mobilizes all the forest insects into an army to help Johnny defeat the giant and rescue the other boys. Johnny and the boys are restored to full size. The film ends with them returning to the scout camp, with the giant shrunk to insect size in a birdcage as a souvenir.