Penelope Poodle (voiced by Robyn Moore) is a very beautiful French poodle. She is pink and wears a magenta dog collar, a matching skirt and purple panties. Despite her beauty, Penelope is a little bit snobbish but she has a good heart. She wants to get to her mistress, Blinky, Nutsy and Flap meet her in a circus where she rides on a rope in the Circus Brothers' Circus. She travels around the world with Blinky, Nutsy, Flap and other animals. She has a diamond necklace, that she was given from her Mistress. She also speaks in a French accent.
Penelope originally lived in Paris, France under the ownership of an unnamed mistress. At some point Penelope was kidnapped and forced to live in a circus under the custody of Basil and Cyril Circus. Her mistress had then moved to a new home and adopted a cat named Fifi as a replacement, but still mourned losing Penelope.
When Blinky and his friends first meet Penelope she is a rope-dancer in the Circus Brothers' Circus. Blinky rescues her along with the other circus animals and she travels with him around the world.
In "Leo Leads the Way" she dressed Nutsy up as a princess.
In "Diamonds Are Forever" the Circus Brothers steal her necklace (thanks to Tico) and she gets herself caught in an attempt to reclaim it, luckily Ling-Ling brings it back (having fooled the brothers and Blinky into thinking she had thrown it into a stream).
In "Baby Elephant Walk" she was actually against helping Kiku get well from a disease at first, but warmed up and became more enthusiastic after persuasions by Nutsy.
In "Double Trouble" she had sore feet due to the long walk in the Amazon jungle.
In "Poisoned Penelope" she is poisoned by the flower called Purple Devil in the Amazon jungle and nearly died from it, but was saved by the Environmentalists.
In "A Dog's Best Friend" she finally returns to Paris and eventually finds her mistress, but her mistress has a cat now. She was originally going to trade her necklace to Maurice for a new home (believing her mistress doesn't miss her at all) but surrenders her necklace to the Circus brothers for Fifi and her Mistress gets her back, Blinky also returns the necklace after trading Cyril's teddy-bear for it and it was here that Penelope bids her rescuers farewell.
- Penelope is the only former circus-animal who is not a natural wild-born animal, instead she is a pet and belong to a human.