Misty's first appearance was in the very first episode of the anime series, Pokémon - I Choose You! In this episode, she first meets Ash after fishing him out of the water when he was trying to get away from Spearow. Ash then takes Misty's bike from her in order to escape the Spearow, which is then wrecked by Pikachu's Thunder Shock. Misty then tracks down Ash and follows him around on his adventures until he can pay her back. She eventually forgets about the bike and becomes one of Ash's closest friends. She is currently the Cerulean City Gym Leader.
In addition to being tomboyishly beautiful, Misty has a slight inferiority complex, mostly due to her three older sisters Daisy (oldest), Violet (second-from-oldest) and Lily (third-from-oldest), who formed the group, "The Three Sensational Sisters" (their synchronized swimming group), and refer to Misty as the "runt" or "weaker one". This complex diminishes over time as Misty grows closer to Daisy. Until Misty became the Cerulean City Gym Leader, her sisters were the ones who ran the gym, although when Ash came along to get his Cascade Badge, it was Misty who battled him for it. Despite being the youngest of four sisters, Misty's the smartest and also the most mature and responsible of her sisters, with Violet being the next smartest and next most mature and responsible. Even if Misty's sisters can be kind of mean to her, they really do care about her, they just aren't very good at showing it sometimes. Misty specializes mainly in Water-type Pokémon, which are her favorite type, but she's had other types, and she also deeply admires Lorelei, an Ice-type Pokémon trainer. Misty aims to become a world-class trainer, and her well-trained Pokémon reflect her aspirations of becoming so. Her least favorite type of Pokemon are Bug-type Pokémon, as she has a deep-rooted fear of them. However, Misty's fear lessens over the course of the series, but not to the point where she's totally gotten over it, as shown in A Real Cleffa Hanger. As the series continues, she gradually shows herself to be kind and sensible. She restrains Brock from girls, often pulling him away by the ear.
In the series, Misty first is seen wearing a yellow tank top with red suspenders and light blue denim shorts. Her shoes were colored red and white, with a yellow streak in the middle. Misty's personality in the anime can be very complex at times. She can be very nice to her friends but will then quickly change moods in a short amount of time, particularly anger. She is quite proficient in anything having to do with water as can be seen in certain episodes where she has ability in fishing, swimming, and overall Water Pokémon training. Even though she wanted to stay with Ash, Misty felt obligated to watch over the Gym. The two went their separate ways, and Misty became the official Gym Leader at Cerulean City's Gym.
Misty's second costume is later shown after she meets up with the main group in Hoenn, after she had already left. Here she wears a yellow, sleeveless jacket with a blue collar, and a large blue button. She also wears short, form-fitting shorts that is connected to a crimson colored upper body piece, which may be a swimsuit. Her shoes are now light and burnt orange.
Misty has only appeared as a guest star in the remainder of the Pokémon series. In one of her cameo appearances in theAdvanced Generation series, her Togepi evolves into Togetic. Misty releases Togetic after it chooses to stay behind to defend the Mirage Kingdom. After Ash finishes competing in the Hoenn League, he returns home to Pallet Town to find Misty waiting for him. However, she isn't the only one to rejoin Ash, as May, Max, and Brock join him as well for theBattle Frontier challenges. She travels with Ash for two episodes, and shortly after returns to her Gym.
- English: Rachael Lillis (Seasons 1 - 8, Movies 1 - 5)
- English: Michele Knotz (The Mastermind of Mirage Pokémon - present)
- Japanese: Mayumi Iizuka
- Swedish: Anna Book
- Norwegian: Anine Kruse
- German: Angela Wiederhut
- Dutch: Marlies Somers
- Italian: Alessandra Karpoff
- Spanish: Miriam Valencia, Pilar Gonzalez-Aguado
- Czech: Eva Spoustova
- Greek: Michaella Antoniou
- Danish: Lulu Jacobsen (Season 1-2), Simone Drechsler (Season 7), Annevig Schlede-Ebbe (Season 6-8)
- Canadian French: Kim Jalabert
- Arabic: Majd Zaza
- Catalan: Nina Romero
- Polish: Iwona Rulewicz
- Russian: Tatiana Zinovenko
- Filipino: Candice Arellano
- Mandarin: Lin Kailing, Long Xianhui, Xie Jiaojuan, Lin Meixiu
- Brazilian Portuguese: Marcia Regina
- Serbian: Maja Dakic
- Croatian: Jasna Palic-Picukaric, Olga Pakalovic
- Korean: Chi Mi-Ae
- French: Fanny Roy
- Thai: Sansanee Wattananumomchan
- Hebrew: Adi Ben Israel, Na'ama Ozen, Talia Barkai
- Portuguese: Helena Montez, Paula Pais, Carla, Isabel Ribas
- Iberian Spanish: Xochitl Ugarte
- Turkish: Birtanem Coskun-Candaner
- Despite Misty being a Water-type trainer, she has had some exceptions in the anime which are, Togepi which is a Fairy type, Togetic which evolved from Togepi is a Fairy/Flying type, and Azurill which is a Normal/Fairy type.
- Misty is the first victim of the recurring gag in the anime where Ash's Pikachu destroys every main female character's bike. This gag was also transferred to May and Dawn. However, this gag did not affect Iris, Bonnie, or Serena, because they did not have bikes.
- In the anime, Misty possesses a fear of most Bug-types (although certain bug Pokémon like Ash's Butterfree and Ash's Heracross don't seem to bother her).
- She also has a fear on Gyarados after crawling into its mouth as a baby, despite it is part Water-type. She overcame this fear and she had a Gyarados on her own that knows Flamethrower.
- Misty was the one who originated the gag of pulling Brock away from pretty women. She pulled Brock on the ear every time he flirted with a pretty woman. This gag transferred to Max in Hoenn and then Brock's Croagunk in Sinnoh (though Croagunk used Poison Jab instead of pulling Brock's ear).
- Misty originally called Ash's Caterpie "ugly" and "disgusting"; Ash later attempted to leave Misty only to have her secretly following him. Once it evolved to Butterfree she warmed up to it.
- Misty has three things she has a strong dislike for. They are carrots, peppers, and bugs stated in episode 3 of the first series.
- In the second Pokémon movie, Misty got jealous after Melody kisses Ash, implying that she may have a crush on Ash. However, she denied having a crush on Ash. In fact, Misty's Songstrongly implies this even more.
- Misty is the only female main character to appear in more than one generation as she appeared in Generation I and II as a main character, while May, Dawn, and Iris have appeared in only one generation as a main character.
- Misty adopts a different personality when talking about/interacting with water Pokémon, as noted by Ash in the episode Tentacool & Tentacruel.