They come from the cartoon series The Glo Friends and serves to the evil Ant-king Lord Driver as the court jesters of the Red Ant Army.´ They only appeared in four episodes called:
"Easy Money part 1-2" and "The Quest part 3-4 of 10."
They are two anthropomorphic, brightly colored fire ants who wears each "cap and bells" hat and blue shoes but not any other clothes. They have two arms and legs instead of six and have a long duck-like beak, also having wide eyes with small pupils. They speak with a silly voice and gladly talks about anything.
The Jester sisters/brothers get in troubles often by their own jokes. They are not the brightest and will entertain anyone or themselves. They have no particular personal differences from each other. They are very much the same. They really like to make fun of Nails.
In episode 3 they tried to amuse their king by telling a bad joke about two cockroaches while juggling clubs, they throws them up in the air, finishing the joke with the clubs smashing their heads onto the ground. Lord Driver was not amused by it and tell the guards to send the jesters to the dungeons. They later met The Glo friends (The Good Guys) and The Moligans (The Bad Guys) in the dungeons. They argues, jokes and laugh and one of the jesters makes a joke about how ugly Nails is. She gets angry of course. Nails quickly grabs one of the jesters and asks threateningly on what the ant said. The taken one says that it only was a joke but Nails strangles the ant and manages to convince him/her that she is beautiful. She later drops him/her down. However, they immediately calls her a witch and runs out of reach from her, sticking out their tongues, hurting her feelings. Later on a small group of other Glo Friends are in the ant-palace to rescue their fellow friends. Jester brothers/sisters takes the chance to escape also. They thank the Glo Friends and runs from there, never to be seen again.
Late in the episode "Easy Money Part 1" Jester brothers/sisters went to the party in Moleslavia. Directly from an engagement of the ant palace. A presenter introduces them enthusiastically with a fanfare upcoming. Jester brothers/sisters jumped out of the dark entrance hopping, doing front flips like mad. Not long after they make a high jump but clumsily lands right in a bowl of punch making the drink splash right on Nails's face. Soaking her entirely. Everyone laugh at her which makes her very angry on the Jesters. Jesters brothers/sisters slowly stand up in the bowl wet, muddled and covered with each broken cherry on their heads, which makes them look stupid. Nails takes a close look at them and begin to scream out of anger. Jester brothers/sisters took off the cherries at that moment. She screams to the king that she have never been so insulted in her entire life. King Mole says that the accident was only in good fun. Then Starnose comes while carrying a very large piece of gold that is shaped like his head. He asks the king to buy the jesters for his gold piece. The King immediately makes a deal and calls the guards to send them to the dungeons. While Jester brothers/sisters heard the king's call they became scared and hold their heads. Nails became grateful for Starnose kindness, however, king Mole notice that the gold suddenly change to a rock. He madly asks Starnose on what is going on. The Moligan, The Excavator says that the formula doesn't affect any more. The king becomes mad and crushed the rock to pieces and tells the guards to immediately send them to the dungeons like the jesters. In part 2 one of the Moligans, Roc the crow gently asks king Mole that there's a lack of court fools and he would like to apply. The king says that if he is a friend of Starnose he should be qualified for the job and the king hires him.
Later on Jester brothers/sisters, in prison, asks the guard on what is going on. The guard tells them that the Moligans are frauds. One of the brothers/sisters also asks if they are free to go, thinking that they no longer belongs to the Moligans. The guard tosses the Moligans right in and close the gate. He tells to Jesters brothers/sisters that "orders are orders" and also tells them that they already have been replaced by Roc. On the next day the guards got breakfast for the prisoners and the new, proclaimed jester Roc the crow is there to tell the Moligans some news about that the wizard Molevolio have the recipe for the formula and that his going to make real gold to pay off the Moligans debt so they will be released. Starnose is mad that they have the formula and Roc is on the King's side. Starnose beats him. The guards tell them to stop but start to fight also and other Moligans also fights the guards. While they are fighting Jester brothers/sisters, safely standing on a high ground net, cheering to Nails and the others to beat the guards.
However, while the guards are fighting some of the Glo Friends and Glo Wees escape to start a plan on how to rescue the others. They are going to get some moondrops and dewdrops for the other because they don't have enough energy to glo. Later the Glo Friends and Glowees arrived at Molevolio's laboratory and finds bags of moon-and dewdrops. However the wizard noticed them and casts an explosive spell on them but it missed and hit the wall instead. Perfect timing for them to escaped. They later went to the market disguised to find a friend named Gregorious Rat and he owns a clothing store. They talk about how to get in the castle again, unnoticed and Gregorious suggests that they can be disguised as his assistants - They are going to sell clothes to the King. While they are at the King's room a feather from a pillow slips in the Glo Friend's and Glowee's disguise. It makes them sneeze so hard that their disguise collapse and they starts to run. They run to the dungeons, taking the cell keys from wall and goes in to close the gate. The guards are at their way and scream to them to open the gate.
The Glo friends and Glowees give the other some moon-and dewdrops. The wizard Molevolio comes to blast the gate with his staff and King Mole also comes to witness it, but trips on his own new clothes and starts to fall right on Molevolio causing a big blast and completely destroying the gate. All the prisoners can escape, but the exit is blocked by soil and boulders. Country mouse's cousin Astrologious says that is was caused by an earthquake but the Moligans starts to dig them out. The Glo-Wee Spruce take some pebbles and juggles with them making the soil and stone to fall. The successfully made the entrance.
Gregorius waits outside with two boats for them. The Moligans waste no time to get in their boat but Nails is having trouble to get on. One of the brothers/sisters sits on Nails's shoulder holding her tight. He/She is afraid to get in the boat by him/herself and maybe trip in the water. The other jester managed to get in the boat. Nails tell the others to hold the boat still, but she trips and fall with the head first right in the middle of the boat. The Jester who sat on Nails shoulder also falls with head first with legs pointing at the sky while the other one is looking right down at the water. They all escaped after all.
In the French dub, their gender was changed to females. There are no explanations on why it was changed but there are other facts about why https://tvtropes.org/pmwiki/pmwiki.php/Main/GenderFlip.
They were called Les soeurs Jester. Meaning The Jester sisters.
Known voice actors:
Voiced by Charlie Adler in the English dub.
Voiced by Maria Santander in the Spanish dub.
Voiced by Anders Öjebo and Bertil Engh in the Swedish dub.